As a laboratory manager, you are likely familiar with the pressure to increase your lab’s sample throughput — the ability to process a certain number of samples on a single instrument within an eight-hour shift.
The challenge of increasing the number of samples processed daily while maintaining the highest standards of data accuracy can be complex — especially when needing to balance costs.
For diagnostic labs, one of the most effective ways to achieve better sample throughput is through multiplex panel testing. By simultaneously testing multiple analytes in a single run, multiplex panels enable labs to meet increasing demands while maintaining accuracy and minimizing costs. Below, we discuss practical and actionable methods for increasing your lab’s efficiency without compromising on quality or exceeding your budget.
Understanding Medium- to High-Throughput Systems
In diagnostic labs, throughput is a critical measure of productivity. However, not all labs require the same throughput solution. For medium- to high-throughput settings, choosing the right system can make a significant difference in workflow efficiency and cost-effectiveness.
While some laboratories may prefer “sample-to-answer” instruments — which fully automate processes from extraction to result analysis — these systems may not be the most efficient option for high-throughput settings. Sample-to-answer systems often require continuous sample loading, which increases hands-on time and operational costs.
This approach can also create a high-stress environment when running multiple tests, as it demands constant interaction from lab technicians. Instead, sample-to-answer devices are better suited for STAT labs or urgent care environments where rapid results are critical.
For laboratories handling higher sample volumes, a “batch” panel testing system offers a practical and cost-effective solution. The BioCode® MDx-3000 is specifically designed for multiplex panel testing, making it ideal for medium- to high-throughput settings. This batch panel testing system supports simultaneous processing of up to three diagnostic panels in a single run, providing exceptional flexibility and efficiency by minimizing the number of required samples and maximizing throughput.
Unlike sample-to-answer devices, batch systems — particularly those designed to support multiplex panel testing — reduce the need for continuous sample handling, freeing up technicians to manage other tasks and creating more walk-away time.
How to Improve Sample Throughput
With these challenges in mind, there are four ways to improve sample throughput.
1. Improve Existing Systems and Procedures
Reviewing existing systems, procedures, and technology regularly — and updating as needed — can enhance your lab’s efficiency and sample throughput. Doing so can help identify processes or instruments that slow down the workflow. This is the first step that can help save time and resources, as it is easier to improve or expand on existing systems.
For example, laboratory managers should evaluate whether adding a dedicated multiplex panel testing system like the BioCode® MDx-3000 could enhance throughput. By consolidating multiple assays into a single test run, multiplex panels allow labs to process more samples without increasing reagent consumption or technician workload. For example, running three distinct diagnostic panels simultaneously on a single system can drastically reduce the total number of samples per run required in a shift.
Another option is to optimize the lab’s layout. Lab managers can strategically reorganize equipment to help reduce the time technicians spend moving between equipment so that samples can be processed faster. Consider replacing several “sample-to-answer” devices with a single batching system like the BioCode® MDx-3000 to save space and decrease steps.
Additionally, adjusting procedures like reagent changeovers can help avoid downtime. Labs can schedule maintenance or reagent replacements during off-peak times.
The methods of improvement may look different for each lab, but the goal is to identify and address bottlenecks in the workflow and make the necessary improvements.
2. Automate Processes
Automation is particularly impactful when paired with multiplex panel systems, as it streamlines the handling of complex panels that target multiple analytes. Systems like the BioCode® MDx-3000 automate crucial processes — such as PCR amplification, target capture, and hybridization — while supporting panel specific workflows that reduce hands-on time and increase efficiency.
For example, automated liquid handling systems can be particularly transformative. These systems handle precise liquid transfers and sample preparation steps — such as pipetting and reagent mixing — across multiple samples simultaneously. They help reduce the time and effort required by cutting down on manual intervention. As a result, they may also minimize the risk of human error and accelerate processing speed.
Systems that automate detection workflow and data analysis also decrease the risk of human error and reduce time spent analyzing graphs and interpreting results.
In contrast, labs that rely on manual processes often need to perform additional checks, such as manual pipetting or sample tracking. These processes may decrease throughput and increase the chance of inconsistencies. Automation reduces this burden and allows staff to focus on more complex activities, leading to higher productivity.
For example, once a run is started on the BioCode® MDx-3000 — an automated system — there is a 3.5-hour walk-away time for the lab technician to work on other tasks. They can use this time to prepare another batch of samples for the next run, for example, thus increasing throughput in an 8-hour shift.
Altogether, automation helps labs minimize the potential for human errors. The main goal is to ensure more consistent results, which is vital when processing large volumes of samples.
3. Switch From Singleplex to Multiplex Assays
While singleplexing may be suitable for low-volume labs with single-target detection requirements, it’s generally not efficient for high-throughput laboratories or multi-target testing.
To increase efficiency and facilitate a high throughput, consider switching from singleplex to multiplex assays. Operating a multiplex system can save processing time for several reasons:
- Simultaneous target detection: Multiplex assays allow the detection of multiple targets in a single reaction, eliminating the need to run separate reactions for each target. Rather than filling multiple wells and extending turnaround time, this consolidation maximizes the use of a 96-well plate and reduces the total number of runs required.
- Fewer reagents and pipetting steps: Multiplex panels use fewer reagents and require fewer manual pipetting steps compared to running multiple singleplex assays, which streamlines the workflow.
- Reduced instrument run time: With multiplexing, a single instrument run can generate results for multiple targets simultaneously, as opposed to requiring multiple separate runs for each singleplex assay. This reduces the overall machine time.
- Consolidated sample preparation: Sample preparation steps can be combined when running a multiplex panel, reducing the time spent on separate preparations for individual assays.
- Lower operator involvement: Multiplexing minimizes hands-on time by reducing the number of separate reactions an operator must set up, monitor, and process.
- Streamlined reporting: Multiplex assays can generate comprehensive results in a single report, avoiding the time-consuming task of collating results from multiple singleplex tests.
By addressing all these factors, multiplex assays provide a more efficient, high-throughput solution that is particularly beneficial for busy labs or those handling high sample volumes.
4. Implement Predictive Maintenance for Equipment
One inconspicuous strategy would be to implement predictive maintenance for lab equipment. Keeping lab equipment running at optimal performance is essential for maintaining sample throughput.
Predictive maintenance uses data-driven insights to anticipate equipment issues before they lead to unexpected downtime. Predictive maintenance can monitor key indicators, such as temperature, pressure, or performance metrics. Knowing the status of lab equipment can help labs detect early signs of wear or malfunction and schedule maintenance proactively.
For example, advanced diagnostic equipment like PCR machines or centrifuges may undergo high levels of daily use. Predictive maintenance tools can alert you when performance starts to dip. This prompts labs to quickly address small issues before they become major problems.
It’s a strategy that helps minimize disruptions to your workflow and ensure your equipment is always ready for the next batch of samples — maintaining consistent throughput. This proactive approach may also extend equipment life span and reduce repair costs. Thus, predictive maintenance tools create a more efficient, cost-effective lab environment.
At Applied BioCode Inc., our field engineers and technicians monitor the system health of our products, like the BioCode® MDx-3000, on a weekly basis. We also have remote diagnostic capabilities which reduce the amount of system downtime and travel expenses. Both our monitoring and maintenance services decrease workflow disruptions and unnecessary site visits, keeping productivity on track.
Why Trust Us
Applied BioCode Inc. specializes in medium- to high-throughput diagnostic multiplex panel testing with innovative solutions designed to enhance your lab’s productivity. BioCode® Barcoded Magnetic Bead (BMB) technology — combining “digital barcodes” with molecular chemistry — supports precise and cost-effective molecular diagnostics. BMBs are created via advanced semi-conductor manufacturing processes that minimize lot-to-lot variability.
Our products are designed to enhance multiplex molecular diagnostics by optimizing sample throughput and ensuring precise, cost-effective testing. For example, our user-friendly BioCode® MDx-3000 enables labs to process up to 188 patient samples within an eight-hour shift and run up to three different panels in parallel.
Our multiplex system eliminates the need for multiple instruments to scale up. With features like data masking, an intuitive interface, and post-run decontamination, it’s designed to save time and boost cost-effectiveness. This enables your lab to maximize sample throughput and efficiency, helping you achieve your throughput goals with ease.
Transform Your Laboratory Experience With Applied BioCode Inc.
If you’re ready to enhance your laboratory sample management and maximize molecular diagnostics sample throughput, partner with Applied BioCode Inc. Our high-quality products, including advanced assays and instruments, are designed to enhance your lab’s capabilities and streamline panel testing.
Contact us to learn more about why we’re the ideal choice for cost-effective, high-throughput syndromic panels.
