Mouse Cytokine/Chemokine Multiplex Kits: Pre-Mixed or Custom Panels*

Next Generation Multiplex Cytokine Highlights:


• Digitally Barcoded Magnetic Beads


• Pre-mixed or Custom Panels


• Partial Plates Available – 32 or 96 Test Kits


• Just like ELISA, with Direct Imaging


• No Lasers, No Flow Cells, No Fluids, and No Waste


• Up to 40% lower Prices than Competitive Offerings

Cytokines and Chemokines are a diverse group of soluable proteins, peptides, or glycoproteins which act as chemical regulators or signaling molecules, and help in cell signaling. A multiplex detection panel can be initially used as a screening tool to identify relevant biomarkers that can then be assembled into a custom panel for follow-up studies.


Mouse Cytokine Pre-mixed Multiplex Kits:


Pre-mixed Panels
(All the reagents are supplied as ready-to-use)
Mouse Th1/Th2/Th17 7-Plex Panel

(IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα)

Mouse Th1/Th2/Th17 10-Plex Panel

(GM-CSF, IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A, TNFα)

Mouse Th1/Th2/Th17 14-Plex Panel

(IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22, TNFα)

Mouse Th1/Th2/Th17 18-Plex Panel

(GM-CSF, IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22, IL-23p19, KC, TNFα, TSLP)

Mouse Inflammation 8-Plex Panel

(IFNγ, IL-1α, IL-6, IL-10, IL-12p70, KC, MCP-1, TNFα)

Mouse Inflammation 11-Plex Panel

(IFNγ, IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-23p19, IP-10, KC, MCP-1, TNFα)

Mouse Inflammation 17-Plex Panel

(IFNγ, IL-1α, IL-1β, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-23p19, IP-10, KC, MCP-, MIP-1α, MIP-1β, RANTES, TNFα)

Mouse Chemokine 7-Plex Panel

(Eotaxin, KC, MCP-1, MCP-3, MIG, RANTES, IP-10)


Mouse Cytokine Custom Multiplex Kits


Applied BioCode’s Mouse Custom Multiplex Kits are available to maximize flexibility in experimental design allowing the user to pick and choose their own analytes panel; each analyte in a specific group can be multiplexed together in any combination.

Pricing for multiplex Cytokine Panels are based on the number of plex per panel.

* Cytokine targets in different groups may be multiplexed together but may also have some cross-reactivity. Specificity of all the cytokine targets across different groups have not been validated.


Custom Panels
(All the reagents are supplied as ready-to-use)
Mouse Cytokine/Chemokine Group 1 (16-plex)

Eotaxin, IFN-gamma, IL-1 alpha/iL-1F1, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A/CTLA-8, IP-10, Gro alpha/KC/CINC1/CXCL1, MCP-1/JE/CCL2, MCP-3/MARC/CCL7, MIG/CXCL9, RANTES/CCL5, TNF-alpha)

Mouse Cytokine/Chemokine Group 2 (18-plex)

G-CSF/CSF-3, GM-CSF/CSF-2, IL-1beta/IL-1F2, IL-3, IL-5, IL-7, IL-12/IL-23 p40, IL-13, IL-15, IL-17F, IL-21, IL-22, IL-28A/B/IFN-lambda 2/3, M-CSF/CSF-1, MIP-1alpha/CCL3, MIP-1beta/CCL4, OSM/Oncostatin M, TSLP

Mouse Group 3 (18-plex)

BTC/Betacellulin, CTACK/CCL27, CXCL16, IL-1RA, IL-9, IL-23p19, IL-25/IL-17E, Leptin/LEP, LIF, LIX/GCP2/CXCL5, MCP-5/CCL12, MIP-2/GRO beta/CINC3, MIP-3α/CCL20, VEGF, CD25/IL-2Rα/Ly43/sCD25, CD127/IL-7R/IL-7Rα/sCD127, CD254/RANKL/TNGSF11/OPGL/sCD254, CD257/BlyS/BAFF/TNFSF13B/sCD257

Mouse Group 4 (18-plex)

6Ckine/CCL21/Exodus-2, Activin A, BLC/CXCL13, BDNF, BRAK/CXCL14/MIP-2γ/BMAC, FGF-1/FGF-acidic/HBGF-1, FGF-2/FGF-basic/HBGH-2, FGF-9/HBGF-9/GAF, HGF/HPTA/SF/LEP, IL-11, IL-20/IL-10D, IL-33/NFHEV/DVS27, MDC/CCL22/ABCD-1/STCP-1, MIP-1γ/CCL9/CCL10/MRP-2/CCF18, MIP-3β/CCL19/ELC, OPG/Osteoprotegerin/TNFRSF11B, TARC/CCL17, TIMP-1

Mouse Group 5 (19-plex)

CD10/Neprilysin/sNEP/CALL, A/sCD10, CD14/sCD14, CD27/sTNFRSF7/sCD27, CD54/sICAM-1, CD62E/sE-selectin/ELAM1, CD62L/sL-selectin/LECAM­1, CD62P/sP-selectin/ LECAM-3, CD105/Endoglin/sCD105, CD106/sVCAM-1, CD119/sIFNγR1/IFNγRa, CD120a/sTNFRI/TNFRSF1A, CD120b/sTNFRII/TNFRSF1B, CD121a/sIL-1R1, CD153/sCD30L/TNFSF8, CD154/sCD40L/TRAP/TNFSF5, CD266/sTWEAK-R/Fn14/TNFRSF12a, CD309/sVEGFR2/KDR/Flk-1, RAGE/sRAGE/AGER, VEGFR1/Flt-1/sVEGFR1


Assay Performance:

Based on Applied BioCode Barcoded Magnetic Bead Multiplexed Technology

a. Mouse Th1/Th2 7-plex Panel Standard Curves

Applied BioCode Mouse 7-plex Standard Curves


The Stock Standard concentrations will vary from lot-to-lot; the concentration of each lot is assigned based from their corresponding retention lot.

Specificity: The analytes of the multiplex were assayed individually at 10,000 pg/mL with the Cytokine 7-plex antibody bead kit and all were found to have negligible cross-reactivity.


Human Cytokine Kits >

Rat, Monkey, Canine, and Non-Human Primate Cytokine Kits >

For Ordering:

Please call 562-801-0050, ext. 253, 229,
or email

* Cytokine/Chemokine products are intended For Research Use Only. Not for use in Diagnostic Procedures.

Instrument Requirement:

BioCode 2500 Instrument is needed to run the Applied BioCode Cytokine Kits


Kit Content:

    • Pre-mixed Capture Antibody conjugated on Barcoded Magnetic Beads (BMB)
    • Pre-mixed Biotin Detection Antibody Conjugates
    • Detection Antibody Diluent
    • Standard Mix, lyophilized recombinant proteins
    • Standard Diluent
    • Streptavidin R-phycoerythrin (SAPE)
    • Assay Buffer

(sufficient amount of reagent to run one 96-well microplate)

Mouse Cytokine Panel Individual Reagents

Individual reagents can be purchased separately. Call Applied BioCode Customer service for more information on pricing and availability.

Reagent Description

Mouse Cytokine Panel Individual Reagents

    • Detection Antibody Diluent, 2X
    • Mouse Standard and Control Mix
    • Mouse Standard Diluent, 1X
    • Cell Culture Standard Diluent, 1X
    • Streptavidin R-phycoerythrin (SAPE), 1X
    • Assay Buffer, 1X
    • Wash Buffer, 10X
    • Detection Buffer, 1X