Human Cytokine/Chemokine Kits: Pre-Mixed or Custom Panels*

Next Generation Multiplex Cytokine Highlights:

 

• Digitally Barcoded Magnetic Beads

 

• Pre-mixed or Custom Panels

 

• Partial Plates Available – 32 or 96 Test Kits

 

• Just like ELISA, with Direct Imaging

 

• No Lasers, No Flow Cells, No Fluids, and No Waste

 

• Up to 40% lower Prices than Competitive Offerings

Cytokines and Chemokines are a diverse group of soluable proteins, peptides, or glycoproteins which act as chemical regulators or signaling molecules, and help in cell signaling. A multiplex detection panel can be initially used as a screening tool to identify relevant biomarkers that can then be assembled into a custom panel for follow-up studies.


Human Cytokine Pre-mixed Multiplex Kits:

 

#
Pre-mixed Panels
(All the reagents are supplied as ready-to-use)
1
Human Th1/Th2 10-plex Panel

(IFNγ, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNFα)

2
Human Th1/Th2/Th17 7-Plex Panel

(IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα)

3
Human Th1/Th2/Th17 14-Plex Panel

(IFNγ, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, TNFα, TNFβ)

4
Human Th1/Th2/Th17 17-Plex Panel

(GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-22, IL-33, TNFα, TNFβ, TSLP)

5
Human Inflammation 7-Plex Panel

(IL-1β, IL-1RA, IL-6, IL-8, IL-10, IL-12p70, TNFα)

6
Human Inflammation 11-Plex Panel

(IFNγ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IL-27, IP-10, MCP-1, TNFα)

7
Human Inflammation 18-Plex Panel

(IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1α, MIP-1β, and TNFα)

8
Human Chemokine 6-Plex Panel

(Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10)

9
Human CD8+ T Cell Related Cytokines, 16-plex Panel

(GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, sCD137, sFasL, TNFα)

10
Human IGF I and IGF II 2-Plex Panel

(IGF I and IGF II)

 


Human Cytokine Custom Multiplex Kits

 

Applied BioCode’s Human Custom Multiplex Kits are available to maximize flexibility in experimental design allowing the user to pick and choose their own analytes panel; each analyte in a specific group can be multiplexed together in any combination.

Pricing for multiplex Cytokine Panels are based on the number of plex per panel.

* Cytokine targets in different groups may be multiplexed together but may also have some cross-reactivity. Specificity of all the cytokine targets across different groups have not been validated.

 

#
Custom Panels
(All the reagents are supplied as ready-to-use)
1
Human Group 1 (17-plex)

(Eotaxin/CCL11,IFN gamma, IL-1beta, IL-1RA, IL-2, IL-4, IL-6, IL-8/CXCL8, IL-10/CSIF, IL-12p70, IL-17A/CTLA-8, IL-22/IL-TIF, IP-10/CXCL10, MCP-1/JE/CCL2, MCP-3/MARC/CCL7, RANTES/CCL5, TNF alpha)

2
Human Group 2 (20-plex)

(G-CSF/CSF-3, GM-CSF/CSF-2, IFN-alpha 2, IL-1alpha/IL-1F1, IL-3, IL-5, IL-7, IL-11, IL-12/IL-23p40, IL-13, IL-17F, IL-20/IL-10D, IL-21, IL-28A/IFN-lambda 2, IL-29/IFN-lambda 1, IL-33, M-CSF, MPO, TSLP, VEGF-A)

3
Human Group 3 (20-plex)

(Fractalkine/CX3CL1, IL-9, IL-15, IL-16/LCF, IL-17C, IL-23, IL-24, IL-25/IL-17E, IL-27, IL-31, IL-34, I-TAC/CXCL11, LIF, MIP-1alpha/CCL3, MIP-1 beta/CCL4, SM/Oncostatin M, SCF/MGF/KITLG, TIM-1/KIM-1/HAVCR, TNF-beta/TNFSF1/LTA, VEGF-D)

4
Human Group 4 (19-plex)

(ANGPT-1/Angiopoietin-1, BDNF, beta-NGF/NGFB, EGF, EPO/Erythropoietin, FGF basic/FGF-2/HBGH-2/Prostatropin, GASP-1/WFIKKNRP, HGF/Hepatopoietin A, IFN beta, Insulin, Leptin/LEP, MIF, OPG/Osteoprotegerin/TNFRSF11B, OPN/Osteopontin, PDGF-AB, Resistin/ADSF, Survivin/BIRC5, TGFalpha/TGF-type I/ETGF, TPO/Thrombopoietin)

5
Human Group 5 (19-plex)

(CD14/sCD14, CD105/Endoglin, CD106/sVCAM-1/sCD106, CD120a/sTNFRI/TNFRSF1A, CD137/s4-1BB/TNFRSF9, CD153/CD30 Ligand/sCD30L/TNFSF8, CD154/CD40L/sCD40L, CD178/sFas Ligand/TNFSF6/sCD95L, CD25/IL-2RA/sCD25, CD254/sRANKL/TNFSF11/TRANCE, CD256/APRIL/TNFSF13, CD257/BlyS/BAFF/TNFSF13B, CD258/sLIGHT/TNFSF14, CD263/sTRAIL-R3/DcR1/TRID/TNFRSF10c, CD309/sVEGFR2/KDR/Flk-1, CD31/sPECAM-1, CD324/sE-Cadherin/CDH1, CD54/sICAM-1, CD62E/sE-selectin/ELAM1)

6
Human Group 6 (20-plex)

(6Ckine/CCL21, BCA-1/CXCL13, BRAK/CXCL14, CCL28, CTACK/CCL27, CXCL16, ENA-78/CXCL5, Eotaxin-2/CCL24/MPIF-2, CD121b/IL-1RII/sCD121b, CD126/IL-6Rα/IL-6R alpha/sCD126, CD130/Gp130/sCD130, CD30/TNFRSF8/sCD30, CD120b/TNFRII/TNFRSF1B/sCD120b, MCP-4/CCL13/SCYA13, IGFBP-1, IGFBP-7, MDC/CCL22/SCYA22, MIP-1δ/CCL15/SCYA15, MIP-3α/CCL20, MIP-3β/CCL19/SCYA19)

7
Human Group 6 (20-plex)

(6Ckine/CCL21, BCA-1/CXCL13, BRAK/CXCL14, CCL28, CTACK/CCL27, CXCL16, ENA-78/CXCL5, Eotaxin-2/CCL24/MPIF-2, CD121b/IL-1RII/sCD121b, CD126/IL-6Rα/IL-6R alpha/sCD126, CD130/Gp130/sCD130, CD30/TNFRSF8/sCD30, CD120b/TNFRII/TNFRSF1B/sCD120b, MCP-4/CCL13/SCYA13, IGFBP-1, IGFBP-7, MDC/CCL22/SCYA22, MIP-1δ/CCL15/SCYA15, MIP-3α/CCL20, MIP-3β/CCL19/SCYA19))

8
Human Group 8 (18-plex)

(BMP-2, BMP-4, Cystatin C, FGF-1/FGF-acidic, FGF-19, Flt-3 Ligand/Flt-3L/Flk-2L, GDF-15/MIC-1/PTGFB/PLAB/PDF/NAG-1, Granzyme B, IGFBP-3, IGFBP-5, IGFBP-6, Lipocalin-2/NGAL, PAPP-A, RAGE/AGER/sRAGE, SLPI/ALP/BLPI/CUSI/HUSI-1/MPI, ST2/IL-1R4/IL-1RL1/IL-33R, TIMP-1, TIMP-2)

 


Assay Performance:

Based on Applied BioCode Barcoded Magnetic Bead Multiplexed Technology

a. Human Th1/Th2 7-plex Panel Standard Curves

Applied BioCode 7-plex Human Cytokine Standard Curves

 

b. Human Th1/Th2 17-plex Panel Standard Curves

Applied BioCode 17-plex Human Cytokine Standard Curves

 

c. Human Th1/Th2 7-plex Assay Performance Characteristics
  IL-2 IL-4 IL-6 IL-10 IL-17A INF-γ TNF-α
Standard Curve Range (pg/ml) 0.6 - 2300 0.3 - 1200 0.5 - 2200 1.1 - 4500 0.5 - 2000 0.4 - 1500 0.5 - 2200
Sensitivity-LOD (pg/mL) 0.4 1.2 1.3 0.5 0.5 0.6 1.5
Specificity (Cross Reactivity) < LOD < LOD < LOD < LOD < LOD < LOD < LOD
Precision, Intra (%CV) <20 <15 <15 <15 <20 <15 <15
Precision, Inter (%CV) <20 <15 <15 <20 <20 <15 <15
Standard Curve Recovery 80 – 120% 80 – 120% 80 – 120% 80 – 120% 80 – 120% 80 – 120% 80 – 120%

 

 

The Stock Standard concentrations will vary from lot-to-lot; the concentration of each lot is assigned based from their corresponding retention lot.

Specificity: The analytes of the multiplex were assayed individually at 10,000 pg/mL with the Cytokine 7-plex antibody bead kit and all were found to have negligible cross-reactivity.

 

Mouse Cytokine Kits >

Rat, Monkey, Canine, and Non-Human Primate Cytokine Kits >


For Ordering:

Please call 562-801-0050, ext. 253, 229,
or email


* Cytokine/Chemokine products are intended For Research Use Only. Not for use in Diagnostic Procedures.

Instrument Requirement:

BioCode 2500 Instrument is needed to run the Applied BioCode Cytokine Kits

 

Kit Content

    • Pre-mixed Capture Antibody conjugated on Barcoded Magnetic Beads (BMB)
    • Pre-mixed Biotin Detection Antibody Conjugates
    • Detection Antibody Diluent
    • Standard Mix, lyophilized recombinant proteins
    • Standard Diluent
    • Streptavidin R-phycoerythrin (SAPE)
    • Assay Buffer

(sufficient amount of reagent to run one 96-well microplate)


Human Cytokine Panel Individual Reagents

Individual reagents can be purchased separately. Call Applied BioCode Customer service for more information on pricing and availability.


Reagent Description

  • Detection Antibody Diluent, 2X
  • Human Standard and Control Mix
  • Human Standard Diluent, 1X
  • Cell Culture Standard Diluent, 1X
  • Streptavidin R-phycoerythrin (SAPE), 1X
  • Assay Buffer, 1X
  • Wash Buffer, 10X
  • Detection Buffer, 1X